Journal: The Analyst

Article Title: Portable electroanalytical nucleic acid amplification tests using printed circuit boards and open-source electronics †

doi: 10.1039/d2an00923d

Figure Lengend Snippet: Schematics and results of the NAAT. (A) Schematics of the RPA followed by a SHA with electrochemical detection: (1) RPA is performed in solution. The RPA product is a dsDNA sequence with specific ssDNA tails at each end (due to the use of tailed primers); (2) the RPA amplicon hybridizes with the capture probe. After a washing step, the HRP-labelled reporter probe hybridizes with the RPA product; and (3) once TMB/H 2 O 2 enzymatic substrate is added, reduction of TMB is measured by chronoamperometry. (B) Representative gel electrophoresis confirming the successful RPA amplification of the tailed target (148 bp dsDNA + 35 bp ssDNA). (C) Chronoamperometric results using Autolab and the portable potentiostat for positive (1 pM target synthetic DNA) and blank (nuclease-free water) samples for gold working electrode 1 (WE1) and 2 (WE2). Chronoamperometry aggregated data showing a statistically significant between groups ( n = 5) at p -value ≤0.05 (unpaired student's t test).

Article Snippet: Electrochemical DNA biosensors appear well suited for POC testing since they facilitate high level miniaturization, compatibility with microfluidic systems, and integration into portable devices, thus precluding the utilization of optical parts such as those found in qPCR thermocycles or in gel-doc instruments.

Techniques: Sequencing, Amplification, Nucleic Acid Electrophoresis